Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.
If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:
- deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
- glue on some transcriptional activators: makes the cell express a gene
- glue on some fluorescent proteins: makes your DNA glow, lets you see where it is
Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high
Genetic engineering has never been this cute
I dunno. For hundreds of thousands of years we’ve been selectively breeding puppies.
Puppies
In a way one could argue genetic engineering is one of the oldest sciences ever. And it started with dogs, or maybe agriculture, depends on what one’s definition of science is.
I want to >!fuck!< CRISPRi
Can’t help it, it’s most compatible
Your spoiler failed
Your
spoilertranscription repressor failedFTFY 👍
Sadly there are two spoiler types i know of :/
for me the spoiler works because i use the “second one?”
“Did you know…”
“…that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of…”
I prefer meowscarada.
Y’all need Jesus. And an assigned FBI agent.
It’s a drawing! It’s fiction! And if you come with the “normalizing” argument, then your “Y’all need Jesus” is normalizing the christofascism that will destroy this world.
Y’all need therapy. Mostly for taking a joke waaaaaaay too seriously.
